[Histonet] Tendon processing

2015-01-12 Thread Andrew Prior
Is anyone willing to share their wax processing schedule for tendon samples? I work on human and porcine tissues so the tendons are quite large , cutting transverse and longitudinal sections for HE and PicroSirius Red staining. We have a protocol at the moment, but are looking to improve it. I

[Histonet] Tendon Processing

2012-06-25 Thread Andrew Prior
Morning all, I am taking over some work on tendon tissue and would like some advice regarding processing schedules. The tissue is 3-5mm thick cross sections from large tendons and is formalin fixed, paraffin wax embedded. At the moment the sections (although flat and wrinkle free) appear

[Histonet] Tendon processing question

2010-01-21 Thread Joel Reichensperger
We are looking for the best way to process tendon (specifically rabbit) that has been severed and then repaired. Currently they are removing the tendon, rinsing it in PBS and then putting it in a cassette in formalin. The problem I am having is that there appears to be some calcification or