Good Morning Histonetters!
I am looking for advice on my thionin staining recipe and protocol. The
recipe I use currently ends up being just under 1% Thionin. It is made by
mixing 80ml 1M acetic acid and 14.4ml 1M NAOH to a pH of 4, and then adding
305.6ml 1.3% stock thionin. When I used this
Simple CV or Thionine method:
Cresyl Violet or Thionine0.1 gram
Distilled water100 ml
when dissolved add
10% acetic acid0.5 ml
*or *
glacial acetic acid100 µl
1. Bring paraffin sections to water as above, frozen or cryostat
sections are rinsed in water.
2. Stain 2-10 minutes in 0.1%
