Hi all,
I'm looking for a protocol to counterstain mouse CFU-F grown in tissue
culture**. The protocols I've found just say they counterstained with this
dye, don't say what it's dissolved in, sometimes list the percentage of it,
and never list the time they stained for. The only full protocol I f
To get consistent staining you must standardize your procedure.
I make 1% aqueous Tol. Blue and 2% aqueous borax (sodium tetraborate) in
separate containers.
Each day I mix equal parts of each solution so I always use fresh stain.
My final conc. of stain is 0.5%, doubling to 1% makes no differe
Hello. Our lab has had highly variable results using toluidine blue to
stain 1.5 micron thick cross sections of plastic embedded nerve tissue
from both mouse and human nerve biopsy. It seems like the stain is
darker in the biopsy tissue than the mouse but both sometimes stain
equally poorly. We ha
