Re: [Histonet] Toulidine blue stain

2009-05-13 Thread Geoff McAuliffe
I have always liked a very simple method from an older edition of Humanson's book. 1. Bring paraffin sections to water. 2. Stain in 0.1% Toluidine Blue in distilled water, 2-5 minutes 3. Rinse thoroughly in distilled water. 4. Dehydrate in 2 changes of acetone, 5 minutes each. Dehydrating in al

[Histonet] Toulidine blue stain

2009-05-08 Thread Amos Brooks
From: "Jennifer Campbell" Subject: [Histonet] Toulidine blue stain To: Message-ID: <5658cbdb9eae6545abe50d2563d81bf821c...@vdxserver01. vdxpathology.local> Content-Type: text/plain; charset="US-ASCII" Hi everyone, I'm trying to find a protocol for

[Histonet] Toulidine blue stain

2009-05-08 Thread Jennifer Campbell
Hi everyone, I'm trying to find a protocol for a toulidine blue stain for paraffin sections. I've looked in different text books and online and can't seem to find anything helpful. Does anyone have a good protocol for this? I need to order the stain rather soon but, first I want to know how mu