I have always liked a very simple method from an older edition of
Humanson's book.
1. Bring paraffin sections to water.
2. Stain in 0.1% Toluidine Blue in distilled water, 2-5 minutes
3. Rinse thoroughly in distilled water.
4. Dehydrate in 2 changes of acetone, 5 minutes each. Dehydrating in
al
From: "Jennifer Campbell"
Subject: [Histonet] Toulidine blue stain
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Hi everyone,
I'm trying to find a protocol for
Hi everyone,
I'm trying to find a protocol for a toulidine blue stain for paraffin
sections. I've looked in different text books and online and can't seem
to find anything helpful. Does anyone have a good protocol for this? I
need to order the stain rather soon but, first I want to know how mu