Re: [Histonet] Trichrome troubleshooting

2015-07-01 Thread Bernice Frederick
[mailto:l...@premierlab.com] Sent: Tuesday, June 30, 2015 3:30 PM To: Suzanne Martin; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome troubleshooting Suzanne How many times have you used the kit and reagents, I did look up how the kit works but the trichrome stain can be tricky

Re: [Histonet] Trichrome troubleshooting

2015-07-01 Thread Terri Braud
Please clarify if you are referring to the Aniline blue, or the Hematoxylin blue. I, also, have never heard of using Gills in association with any Trichrome stain, but as a long time user and educator of using a one-step (Gomori's) trichrome stain (which is the Leica blue collagen stain), if

Re: [Histonet] Trichrome troubleshooting

2015-06-30 Thread Gayle Callis
@lists.utsouthwestern.edu Subject: [Histonet] Trichrome troubleshooting Hi all, We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills. Most of the small bowel controls have seen improvement but patient tissue is not... strange

[Histonet] Trichrome troubleshooting

2015-06-30 Thread Suzanne Martin
Hi all, We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills. Most of the small bowel controls have seen improvement but patient tissue is not... strange. We have tried lessening the time in Gills, adding time for the

Re: [Histonet] Trichrome troubleshooting

2015-06-30 Thread Elizabeth Chlipala
Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: Suzanne Martin [mailto:smar...@lcpath.com] Sent: Tuesday, June 30, 2015 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome troubleshooting Hi

Re: [Histonet] Trichrome troubleshooting

2015-06-30 Thread Michael Ann Jones
: Suzanne Martin [mailto:smar...@lcpath.com] Sent: Tuesday, June 30, 2015 12:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Trichrome troubleshooting Hi all, We are having trouble troubleshooting our trichrome. It is too blue. We are using Leica's kit with the Weigerts iron with Gills

Re: [Histonet] Trichrome troubleshooting

2015-06-30 Thread Gayle Callis
-Original Message- From: Elizabeth Chlipala [mailto:l...@premierlab.com] Sent: Tuesday, June 30, 2015 2:30 PM To: Suzanne Martin; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome troubleshooting Suzanne How many times have you used the kit and reagents, I did look up how