Hi there, I´m trying to trim ovine foetal brain (90 days of gestation). The brain is too soft and there is no way to get a proper slice. It has been in buffered 10% formalin for three weeks but still too soft. When you take the brain out of the pot and put into the trimming table, it just kind of spill over the place. We have tried to embed the brain in 7.5% gelatin, which helps to keep the shape of the brain before trimming, but as soon you cut the slice, the centre of the tissue just melt away. I was wondering if there is any post fixative solution we can try. May be Bouin´s?
Any suggestion would be greatly appreciated! Cheers Julio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
