[Histonet] Trophic factors staining on brain sections

Hi all to stain out the trophic factors on brain sections, such as BDNFm, IGF1, NGF, CNTF, GDNF will these molecules "leak" out during staining? so I should not use triton and try to shorten the time of staining? Or I need a long time incubation to stain out the "low" concentration small molecu

[Histonet] Trophic factors staining on brain sections

Hi all to stain out the trophic factors on brain sections, such as BDNFm, IGF1, NGF, CNTF, GDNF will these molecules "leak" out during staining? so I should not use triton and try to shorten the time of staining? Or I need a long time incubation to stain out the "low" concentration small molecu