FW: [Histonet] Troubleshooting Masson Trichrome Stain

2014-02-11 Thread Elizabeth Chlipala
gmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anna Coffey Sent: Tuesday, February 11, 2014 12:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Troubleshooting Masson Trichro

[Histonet] Troubleshooting Masson Trichrome Stain

2014-02-11 Thread Anna Coffey
Hello Histonetters, We regularly perform the Masson Trichrome stain in our lab and recently have been noticing some inconsistent results. The main issue seems to be that the Biebrich Scarlet is not being adequately removed by the PTA/PMA, as many of the areas containing collagen are showing up as