Joanne,
I do not think you should expect to get perfectly uniform IHC staining in
breast core biopsies every time. At our hospital, we prefer to avoid doing
hormone receptor stains on breast cores because they are not as "robust" as
a lumpectomy or mastectomy specimen.
The unevenness could be due
My first 2 items to check whenever I have uneven IHC staining are (1)
Inadequate deparaffinization, (2) bad lot of charged slides - yes this can
cause terribly streaky or spotty staining, and since you are using the Bond,
perhaps there was an issue with coverplate placements.
Just things to cons
