Hi Sharon,
I use a 1.5% solution for bone marrow cases and bloody tissue. It does also
work well for GI. I've never had an issue with it affecting any of my IHC.
An alternative though (when I can't get my hands on ammonium hydroxide) is
to put these blocks in warm water (I'm careful about the wa
...@lists.utsouthwestern.edu] On Behalf Of
Sharon.Davis-Devine
Sent: Monday, December 27, 2010 3:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Use of ammonia water
Happy Holidays Histonetters, I have a question for you. Do any of you
use ammonia water to soften the blocks before cutting? If so, does this
Happy Holidays Histonetters, I have a question for you. Do any of you use
ammonia water to soften the blocks before cutting? If so, does this have any
effect on IHC staining? Any help will be greatly appreciated. Thanks.
Sharon Davis-Devine, CT (ASCP)
Cytology-Histology Supervisor
Carle Founda
