Re: [Histonet] Use of sodium borohydride in immunofluorescence

2017-03-22 Thread Lucie Guernsey via Histonet
Hi Bob, Yes, thanks for looking out! We're very careful with it (used only in the fume hood and we collect the waste for pickup) and right now I'm just updating protocols. So many of them are outdated and missing steps or helpful info. Thank you, also, for the best wishes. Lucie On Wed, Mar

Re: [Histonet] Use of sodium borohydride in immunofluorescence

2017-03-22 Thread Bob Richmond via Histonet
> > Lucie Guernsey at UC San Diego asks about sodium borohydride: >>I'm currently going over IHC SOPs that were written by lab members that have long ago moved on to other jobs. One inconsistency that I've come across is the use of 0.5 % sodium borohydride in immunofluorescence.<< Does your

[Histonet] Use of sodium borohydride in immunofluorescence

2017-03-20 Thread Lucie Guernsey via Histonet
Hi, I'm currently going over IHC SOPs that were written by lab members that have long ago moved on to other jobs. One inconsistency that I've come across is the use of sodium borohydride in immunofluorescence. Our protocols state that a '0.5% sodium borohydride solution should be used to block