[Histonet] Using GEWF solution and IHC staining

2010-07-27 Thread Hayes, Randi (HorizonNB)
At a recent conference, our PA learned of using GEWF (glacial acetic acid, ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph Node retrieval in Colorectal Cancer resections. Although a good idea, I'm wondering how safe it is to use when staining for IHC. Does anyone have

Re: [Histonet] Using GEWF solution and IHC staining

2010-07-27 Thread Patrick Laurie
Sounds like to me that it might involve another revalidation of each one of your antibodies. Good luck On Tue, Jul 27, 2010 at 11:03 AM, Hayes, Randi (HorizonNB) randi.ha...@horizonnb.ca wrote: At a recent conference, our PA learned of using GEWF (glacial acetic acid, ethanol, distilled

Re: [Histonet] Using GEWF solution and IHC staining

2010-07-27 Thread Mark Tarango
Ethanol/alcohol is what will process the specimen. If the tissue is fixed would it really matter that the tissue came into contact with ethanol? Mark On Tue, Jul 27, 2010 at 11:03 AM, Hayes, Randi (HorizonNB) randi.ha...@horizonnb.ca wrote: At a recent conference, our PA learned of using GEWF