Hi, I’m looking for some advice on using LR white on an osmium stained tissue. Unfortunately, I don’t have access to an ultra-microtome or diamond knives.
Has someone here tried sectioning LR white with tungsten carbide knives on a motorized rotary microtome? Are there any sectioning artifacts that I might see? Also, LR white seems to be available in three grades - soft, medium and hard. Is the soft grade recommended with my setup? Thank you, Chaitanya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet