This is not a good practice and can lead to inconsistent results.Always use
freshly prepared DAB sol. to finally be able to see the end product of the
costly and important IHC procedure, it is worth it.René
On Monday, April 25, 2016 6:27 PM, Andrea Calhoun via Histonet
Hi Listers!
Has anyone ever tried re-using DAB? Not like week or day old DAB, but using the
same DAB solution for more than one rack of slides? Anyone know the length of
time DAB is activated for before becoming inactivated, or quenched? Safe to
do, if more H202 is added?
After antibody