Re: [Histonet] Vectastain elite ABC kit- multiple dips ok?

2010-09-29 Thread Geoff McAuliffe
Teresa Iglesias wrote: Hi all, I have three 24-well plates with three brain sections in each well. For IHC, do I have to prepare three different/fresh ABC dips for each 24-well screen plate or can I incubate (30min) each successive screen plate into the already used dip of ABC? I would not.

[Histonet] Vectastain elite ABC kit- multiple dips ok?

2010-09-28 Thread Teresa Iglesias
Hi all, I have three 24-well plates with three brain sections in each well. For IHC, do I have to prepare three different/fresh ABC dips for each 24-well screen plate or can I incubate (30min) each successive screen plate into the already used dip of ABC? Do I have to leave it in longer to allow