Hi Margaret
You perform the Alcian Blue first but as with Movatt's pentachrome you need to
treat the sections after AB with alcoholic ammonia for an hour to convert the
AB to Monastral Blue as the AB is soluable in picric acid and the Monastral
blue resistant. Let me know if you need a method
We want to try the double stain alcian blue Van gieson. Do you stain for the
alcian blue first and then the Van gieson? We do both stains but I am unsure
of how to put them together.
Margaret Perry
South Dakota State University
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Histonet mailing
Alcian Blue first
- Original Message
From: Perry, Margaret margaret.pe...@sdstate.edu
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Fri, March 12, 2010 9:30:40 AM
Subject: [Histonet] alcian blue van gieson stain
We want to try the double stain alcian
