RE: [Histonet] another Movats question (paraffin)

2013-05-29 Thread Molinari, Betsy
, 2013 6:40 PM To: Molinari, Betsy Subject: Re: [Histonet] another Movats question (paraffin) Any chance this is a new batch of alcian blue? From where did you purchase it? I have a theory, but would like some more information first. Peggy A. Wenk, HTL(ASCP)SLS Lee Peggy Wenk -Original

Re: [Histonet] another Movats question (paraffin)

2013-05-29 Thread Lee Peggy Wenk
What happens if you skip the ammonium hydroxide? Peggy Wenk -Original Message- From: Molinari, Betsy Sent: Wednesday, May 29, 2013 7:45 AM To: Lee Peggy Wenk ; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] another Movats question (paraffin) Hi Peggy, No, this is a batch I

[Histonet] another Movats question (paraffin)

2013-05-28 Thread Molinari, Betsy
Hi, The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been running Movats for years and this is a new problem. I have checked lot #’s everything is the same. The solution is 1% pH 2.7. The slides are in solution for 20 mins, rinse for 5 mins in tap H2O, then

Re: [Histonet] another Movats question (paraffin)

2013-05-28 Thread koellingr
- Original Message - From: Betsy Molinari bmolin...@texasheart.org To: Histonet@lists.utsouthwestern.edu Sent: Tuesday, May 28, 2013 8:47:19 AM Subject: [Histonet] another Movats question (paraffin) Hi, The Alcian Blue in my Movats is very faint or seems to completely disappear. I have been