Hello 'netters, hope you all had a good weekend.
We are having some difficulties with the pressure cooker chambers we use for
antigen retrieval. What different methodologies would be recommended? If we
went to a different methodology for retrieval, would each antigen have to be
revalidated? Ho
Lynn, I use the Decloaker and also stain for BVD, so will get in contact
with you later this afternoon.
Jan Shivers
University of Minnesota Veterinary Diagnostic Lab
St. Paul, MN 55108
On Thu, May 30, 2013 at 9:54 AM, Burton, Lynn wrote:
> If anyone out there is using a decloaker for antigen r
Is this with an older tissue sample? We don't do antigen retrieval, but we do
have a proprietary technique for tissue restoration which can help with
problematic IHC studies, especially in older FFPE tissue samples.
See here for some examples:
http://hematoxylin-eosin-tales.blogspot.com/2013/04
If anyone out there is using a decloaker for antigen retrieval, when doing ihc
staining, would you please contact me on my e-mail address? I have been
struggling with a BVD test for months. It is not working consistently and I
want to explore using this method along with our Benchmark XT stainer
test
immediately after HIER. Maybe that is why it has not happened to me.
Just a thought!
René J.
--- On Tue, 10/20/09, Connolly, Brett M wrote:
From: Connolly, Brett M
Subject: [Histonet] Antigen retrieval question
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 20, 2009, 2:21 PM
Sounds like the retrieved antigen was lost rather than "reversal"
Janet
QIHC/HTL
--- On Tue, 10/20/09, Connolly, Brett M wrote:
From: Connolly, Brett M
Subject: [Histonet] Antigen retrieval question
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, October 20, 2009, 11:21 AM
Has anyone experienced a "reversal" of citrate HEIR unmasking do to a
prolonged delay in continuing the experiment after the retrieval step?
Brett M. Connolly, Ph.D.
Research Fellow, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_co
