Dear Joost, Yes and to read up on this, go to this website for an excellent, well referenced free pdf i.e. Autofluorescence, causes and cures. https://www.google.com/?gws_rd=ssl#q=autofluorescence+causes+and+cures+wrigh t+cell+imaging+facility They also posted a pdf on mounting medias for fluorescence microscopy work.
The pdf will tell you what tissue and cellular components autofluoresce. The authors also provided various methods to reduce or remove autofluorescence of these components but also how to remove aldehyde induced autofluorescence if you are fixing tissues with NBF or PFA. Remember there is no autofluroescence in the Near Infra Red region so one can use an NIR fluorophore, i.e. Alexa 750 (red) or another fluorophore for the NIR region, and even use tissue autofluorescence as a "counterstain fluorescence" However, you cannot see these with the human eye but these photograph beautifully. OR if you have a spectral imaging or confocal capabilities, you can rule out autofluorescence without having to treat the tissue section. If you have difficulty access this pdf, I will send via private email. Gayle Callis HTL/HT/MT(ASCP) Bozeman MT USA -----Original Message----- From: Bruijntjes, J.P. (Joost) via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, February 26, 2016 3:00 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] auto-fluorescence Hi histonetters I hope one of you can help me. I don't have any experience with fluorescence. We are searching for some components and we will use an alexa488 conjugated secondary. Will there be an auto-fluorescence on different organs (brain/spleen/liver)? Greetings Joost _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
