RE: [Histonet] bone marrow aspirations

2013-06-10 Thread Cathy
on the quality of the slides. Cathy Kelowna, B.C. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shea's Sent: Saturday, June 1, 2013 4:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow

[Histonet] bone marrow aspirations

2013-06-01 Thread Shea's
I am also interested in how others are processing bone marrow aspirates. Currently, we let the aspirate clot on a watch glass for about 20 minutes, gentle slide it onto filter paper, roll it around to get rid of the excess fluid, place the clot between sponges, and process as usual. However, I

[Histonet] bone marrow aspirations

2013-05-31 Thread Diana McCaig
Can anyone share with me their process for processing bone marrow aspirations and embedding them in paraffin blocks. Currently ours are being spread out throughout the entire base mold and makes it cumbersome to screen. Has anyone used histogel or making a button and embed that instead of

[Histonet] bone marrow aspirations

2011-03-02 Thread Debra Siena
Hi All, I would appreciate some insight as to how most labs are treating bone marrow smears, in regards to fixation. I am not embarrassed to say that it has been awhile since I worked with them. With the smears are most labs air drying, methanol (alcohol fixation) or spray fixation (pap

Re: [Histonet] bone marrow aspirations

2011-03-02 Thread Rene J Buesa
Either air dry → methanol (most labs) or air dry → PAP fixative (I always preferred methanol). René J. --- On Wed, 3/2/11, Debra Siena dsi...@statlab.com wrote: From: Debra Siena dsi...@statlab.com Subject: [Histonet] bone marrow aspirations To: histonet@lists.utsouthwestern.edu histonet

Re: [Histonet] bone marrow aspirations

2011-03-02 Thread Mark Turner
...@statlab.com Subject: [Histonet] bone marrow aspirations To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Wednesday, March 2, 2011, 10:43 AM Hi All, I would appreciate some insight as to how most labs are treating bone marrow smears, in regards to fixation

Re: [Histonet] bone marrow aspirations

2011-03-02 Thread Jennifer MacDonald
We always air dried them and fixed them in methanol, otherwise they washed off. Debra Siena dsi...@statlab.com Sent by: histonet-boun...@lists.utsouthwestern.edu 03/02/2011 07:49 AM To histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu cc Subject [Histonet] bone marrow