I was having similar issues with frozen rat and human cartilage with the
cryojane. After a lot of trial and error; I have found that the 4X slides and
air-drying overnight has worked extremely well for us. The tissues never fall
off any more!
Good luck,
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Hello, Histo-users,
Follow-up to our original posting about fixed-brain cryosections
falling off of subbed slides. We fished out our Cryo-Jane apparatus, and
using this apparatus has resulted in a great improvement in sections and
getting sections stuck onto the slides. This works pretty
Doug,
Hopefully you are using the CryoJane slides (not the subbed slides) and the UV
glue activator and keeping both slides and tape cold in the cryostat. Be aware
that you can get CryoJane slides with different amounts of adhesive (0.5-4x).
I would suggest going to the 4x slides. When peeling
