[Histonet] brain sections falling off slides during IHC

Hello all, Can any of you all with experience immunostaining brain sections please help. My sections are almost completely washing off the slides. I am using 10% NBF fixed canine brain sections. Embedded in paraffin and sectioned at 4 microns on plus slides with distilled water in the water

Re: [Histonet] brain sections falling off slides during IHC

： [Histonet] brain sections falling off slides during IHC Hello all, Can any of you all with experience immunostaining brain sections please help. My sections are almost completely washing off the slides. I am using 10% NBF fixed canine brain sections. Embedded in paraffin and sectioned at 4

[Histonet] brain sections falling off slides during IHC

A few years ago we also had this problem. The brains were terrible. We found that changing our processing chemicals, especially the wax, more frequently got rid of most of our problems. We use Fisher plus slides. There is a difference in plus slides. I recommend you contact several