You will first need to document the control tissue you are collecting so you
have the ischemic time, fixation time and processing schedule in your records.
Make sure you control your fixation time and don't just grab tissue out of wet
storage, this will save you a lot of grief. Then give each bl
Could anyone share their process of finding and validating control tissues for
IHC?
We currently use tonsil tissue as control tissue for 23 different antibodies
for IHC (CD2, CD3, CD4, etc.).
My current process is to have our grossing personnel submit some extra tissue
from a surgical case.
Does anyone have a good source for obtaining fresh tissue (formalin fixed) for
IHC validation? I would need normal and tumor of various types.
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA 90048
(323) 648-3214 direct
(424) 245-7284 main lab
_
se tissues that are (+) for several procedures. Small intestine
is good for mucin and trichrome. Bone marrow is good for Perl's method.
René J.
From: "Johnston, Amy"
To: "histonet@lists.utsouthwestern.edu"
Sent: Tuesday, January 8, 2013 7:07 PM
Subject: [Histonet] Control
Is there any way to obtain control blocks? We are in the process of starting up
a new histology lab and control slides are a lot more expensive than I
thought:( . I have purchased several boxes already, I was hoping I could find
the others as they should be easy to find. (I tried NSH, they no
Hello All,
I need some help trying to find control tissue for a research project. I need
human breast carcinoma. I work in a Veterinary Diagnostic Lab, so I don't have
any human tissue, but I can exchange various animal tissues for controls. This
would include normal tissue from a variety of
oelle Weaver MAOM, HTL (ASCP) QIHC
> Date: Fri, 2 Nov 2012 11:09:01 -0500
> From: dels...@gmail.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Control tissue
>
> Hi,
> Can anyone tell me if the control blocks we use for special stains and IHC
> need to hav
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter
Sent: Friday, November 02, 2012 10:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Control tissue
Hi,
Can anyone tell me if the control blocks we use for special stains and IHC need
to have
Hi,
Can anyone tell me if the control blocks we use for special stains and IHC
need to have the accession number of the original case they came from on
them? Does this info have to be put on the slides? Are there any CAP
regulations about this? Thank you so much for your help.
Deloris Carter HT(
Hi all. Does anyone have a resource for control blocks? We are in need
of fungus controls and are trying to save the cost of buying slides.
Thanks all.
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc
Sheila,
National Society for Histotechnology has a Tissue Control Bank, If you
con
I am in search of a couple of controls for IHC, ffpe.
One is Human Glioma brain tumor and this one is odd, ffpe rat placenta, can
anyone help me out?
Thank you so much,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720
My apologies, I wasn't ready for the number of responses I received over the
control tissue and might have missed some people. If I didn't contact you back
shoot me another email so I can start working on it.
Control Tissue still Available
Iron/FE
Tonsil
Hpylori
CD15/30
TTF1
p16
AFB
Fungus
ternal controls", use them as a reference.
René J.
--- On Sun, 4/17/11, histot...@imagesbyhopper.com
wrote:
From: histot...@imagesbyhopper.com
Subject: [Histonet] Control Tissue
To: histonet@lists.utsouthwestern.edu
Date: Sunday, April 17, 2011, 10:37 AM
Hi Histonetters!
I have been
Hi Histonetters!
I have been requested to post a question to the group at large: Is there
any way to process a "control tissue" in a tissue processor, with the hopes
of showing that the processor is working up to par? Perhaps someone has a
procedure for this sort of thing? I know we use know
Does anyone have or know where I can get control tissue for the MYCN (2p
24.1) FISH probe assay, an amplified or positive control? Thanks
Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.m
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
beth.villarr...@novartis.com
Sent: Thursday, July 01, 2010 01:51 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] control tissue for Hall's bile stain
Does anyone
Does anyone know a commercial source for control tissue for
bile/bilirubin? Species does not matter.
Thanks!
Beth Villarreal, HT(ASCP)
Novartis Institutes for BioMedical
PCS, Discovery Pathology, US
USCA, 611-7104B
Novartis Institutes for BioMedical
Research, Inc.
100 Technology Square
Cambridg
Hello Histonet,
I am looking for a positive control for the Fluoro Jade Stain consisting
of rodent brain tissue demonstrating neuronal necrosis in the
hippocampus caused by administration of trimethyltin. Does anyone have
access to a stock of controls or can anyone tell me who to contact. I
have
What is anyone using for their control tissue for IHC H-pylori. We have
few and far between gastric resection here and even if we did more, it
is a hit and miss for the organism. We have a policy here to not used
labeled patient tissue blocks as controls.
Thanks,
Mike
We have a plentiful amount (hundreds) of malignat melanoma blocks that we are
willing to trade some for any ER/PR + tissue, colon cancer, or any other
uselful IHC control tissue. Any takers?
This message contains confidential information and is intended only for
The best control is healthy skin with the connective tissue in place but a
tonsil is also great for checking how well your lymph tissue is staining.
Tonsil should be cut thinner just like the lymph nodes so you should use the
tonsil when staining lymph nodes. Make sure the tonsil has connectiv
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