Hi Histonetters
When I soak my slides in xylene to remove the coverslip, do I then need
to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh, and
then H20.
And then drying and re applying permount?
Or can I go straight from xylene to drying to re-coversliping with
permount?
...@seattlechildrens.org
Subject: [Histonet] Coverslip removal question
To: Histonet@lists.utsouthwestern.edu
Date: Friday, October 22, 2010, 3:55 PM
Hi Histonetters
When I soak my slides in xylene to remove the coverslip, do I then need
to rehydrate them by washing them in 100% etoh,95% etoh,70% etoh
I am using aqueous mounting media, faramount, for the first time. The slides
I coverslipped several days ago look dry. Will soaking in water remove the
coverslip?
Margaret Perry HT(ASCP)
Dept of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
