It may help to let your block equilibrate for about 20 minutes in the
cryostat. I've found blocks do not section well if you start sectioning
right away, especially snap frozen ones. Also, do you embed in OCT/sucrose?
A 1:1 OCT: 30% sucrose solution is much softer than just OCT (or whatever
embed
I am new to using a cryostat and am having some issues with my tissue.
1.Every slice seems to shatter and fragment. When I have used a
microtome it was because the tissue was too cold.
2. The tissue itself was flash frozen and so was not fixed and the tissue
did not go through t
