We showed aspestos fibers with EM by using a formvar coated 200 mesh grids. 1
micro liter sample solution was put on the surface of the formwar coated grid.
The grid was dried at room temperature and directly searched with EM.
- Orijinal Mesaj -
Kimden: Rene J Buesa rjbu...@yahoo.com
Dear all
I am a histotechnologist in forensics, Cape Town, South Africa.
I received a request to show asbestos in lung tissue where there is
definite interstitial fibrosis, but the presence of asbestos is not
clear.
Is it possible to reveal asbestos by means of electron microscopy?
Usually
The StainsFile page has some techniques, using polarizing microscopes.
http://stainsfile.info/StainsFile/stain/pigment/asbestos.htm
Asbestos are usually very small fibers, and will not show up in every
section. Cutting thicker sections sometimes helps.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont
Yolanda,
Yes it is, TEM is often used for asbestos detection in lung tissue to identify
chrysotile and/or crocidolite (mesothelioma-associated) asbestos fibers.
There are a lot of publications out there...
Brett
Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck Co., Inc.
PO Box 4,
Many years ago asbestos fibers were demonstrated in hypochlorite digested
tissues. The remaining material was filtered through Milllipore filter, washed
and the fibers observed with polarized light.
The Perl's method will stain no the asbestos fibers (that cannot be stained)
but a reactive
