Dear friends of Histonet,

I have send already a message last week considering problems with thionin 
staining. I got a lot of protocols, thank you for that. We used different kind 
of solutions, but the problem remains, independently of the solution: when our 
samples go through the ethanol (50%, 70%, 90%, 100%) the thionin is completely 
washed of.
Has anyone got the same experience? What to do about it?

regards,

An Eerdekens
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