Thanks very much.
I will extract the lipids first.
2009-02-10
TF
发件人： Geoff McAuliffe
发送时间： 2009-02-10 00:17:29
收件人： tifei
抄送： histonet@lists.utsouthwestern.edu
主题： Re: [Histonet] feulgen stain on brain sections
Dear TF:
Schiff reagent reacts with the lipids (the aldehydes in the
Dear TF:
Schiff reagent reacts with the lipids (the aldehydes in the lipids) in
the frozen sections so the whole section is pink. You could try
extracting the "offending" lipids with alcohols and xylene, then
rehydrate to water and run the reaction again. A better plan is to
search the litera
Hi all, i amusing Feulgen staining to visualize the apoptotic cells on 20 um
brain frozen sections.
The procedure is 5N HCl room temperature for 1.5 hour, wash in cool 1N HCl,
wash in water, Schiff staining room temperature 1 h, wash throughly in
distilled water, counterstain with 1% light green
