Re: [Histonet] hard Tick histology

2020-03-05 Thread Paula Keene Pierce via Histonet
The exoskeletons of insects are made of chitin and contain little calcium. Lysol spray contains a type of phenol which will soften chitin. The phenol is what kills bacteria, mold, and viruses by destroying their outer shells. Try spraying Lysol in a small container so you have a few mls. It will

[Histonet] hard Tick histology

2020-03-05 Thread Mike Tighe via Histonet
Hello, I was wondering if someone might have some expertise on how to prepare a hard-tick for sectioning in paraffin. I have been decalcifying to soften the exoskeleton but not sure if that even helps. I have used acid decalcifying but would EDTA be more appropriate? I have punctured the ticks