Next day, pellet cells and resuspend them in a small amount (100-200 microliters) of Histogel or Agar. When the gel cools cut the microcentrifuge tube open with a scalpel or razor blade. Now you have a nice cell pellet shaped like the bottom of your centrifuge tube, and you can treat it just like any piece of tissue. Pop it in a tissue processing cassette and then to your ethanol dehydrating steps.
We use histogel routinely for clinical specimens. We have found several things that improve the process: 1)after you pellet the cells and decant, use a cotton tip swab to pick up the last little droplet of fluid adjacent to the button. It will hold together much better. 2)after you have added histogel, vortex and centrifuge to pellet. Put tube in ice bath, add a few drops of formalin, and wait a few minutes. With minimal "prodding" the "plug" will essentially pour out of the tube. No need to cut. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, May 13, 2010 7:41 AM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 78, Issue 17 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: FFPE cell pellet prep (JR R) 2. xylene substitutes (Perry, Margaret) 3. RE: AMT Cover (Jones, Laura) 4. RE: AMT Cover (wanda.sm...@hcahealthcare.com) 5. RE: AMT Cover (Jennifer Anderson) 6. RE: RE: AMT Cover (Terri Brown) 7. RE: AMT Cover (Bell, Lynne) 8. RE: AMT Cover (Elliott, Rachel A.) 9. 2010 Louisiana Society for Histotechnology State Meeting - Reservation Extension! (Montina Van Meter) 10. Re: Xylene substitutes (V. Neubert) 11. Gastric biopsies (Cristi stephenson) 12. RE: Forwarding Request for Control Mehtod (Tony Henwood) 13. Re: Gastric biopsies (Brandi Higgins) 14. Re: Gastric biopsies (susanfpl...@aim.com) 15. How do I subscribe? (Michelle MacVeigh-Aloni) 16. job opening for cytology/ FISH and histology supervisor - Florida (dcoj...@tampabay.rr.com) 17. RE: Gastric biopsies (Gill, Caula A.) 18. RE: Are Histotechs considered "exempt" employees? (Heckford, Karen - SMMC-SF) 19. RE: Gastric biopsies (Cynthia Pyse) 20. Re: Gastric biopsies (Rene J Buesa) 21. RE: Gastric biopsies (Weems, Joyce) 22. RE: Gastric biopsies (Joyce Cline) 23. Tilapia Fish Eye (Marquisha Paul) ---------------------------------------------------------------------- Message: 1 Date: Wed, 12 May 2010 10:36:15 -0700 From: JR R <rosenfeld...@hotmail.com> Subject: RE: [Histonet] FFPE cell pellet prep To: <histonet@lists.utsouthwestern.edu> Message-ID: <bay135-w681d7f121be89ca27acdcdb...@phx.gbl> Content-Type: text/plain; charset="iso-8859-1" Here's how I do it. I hate the lens paper method. I transfer suspended cells from a T75 flask to a 50 ml Falcon tube and centrifuge at 400 X g for 5 minutes. Aspirate most of the media so that cells plus media are about 1 ml. Transfer cells plus media to a 1.7 ml microcentrifuge tube. Pellet cells at 400 x G for 5 minutes. Rinse with PBS if needed. Suspend cells in NBF and fix over night. Next day, pellet cells and resuspend them in a small amount (100-200 microliters) of Histogel or Agar. When the gel cools cut the microcentrifuge tube open with a scalpel or razor blade. Now you have a nice cell pellet shaped like the bottom of your centrifuge tube, and you can treat it just like any piece of tissue. Pop it in a tissue processing cassette and then to your ethanol dehydrating steps. Jerry Ricks Research Scientist University of Washington Department of Pathology > Date: Wed, 12 May 2010 06:50:14 -0700 > From: kmerriam2...@yahoo.com > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] FFPE cell pellet prep > > Sorry - I forgot to put a subject line. > > > Good morning, > > I am trying to make some nice FFPE cell pellet blocks, but I seem to lose a > lot of cells along the way (especially when trying to take the cells out of > the tube). We are fixing the cells in NBF, spinning them down, adding 70% > and spinning down again. At that point, I am trying to scoop out the cells > (with a weighing scoop) and wrap them in lens paper for processing. I am > losing a lot of the cells during this step, because the pellet is not quite > solid. Do you think it would be OK to let the cells air-dry a bit and then > take them out for processing? I know this goes against everything I was > taught in histology, but I am really at a loss here. > > Anyone have any hot tips for me? > > Kim > Kim Merriam, MA, HT(ASCP)QIHC > Cambridge, MA > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ The New Busy is not the old busy. Search, chat and e-mail from your inbox. http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_3 ------------------------------ Message: 2 Date: Wed, 12 May 2010 12:42:57 -0500 From: "Perry, Margaret" <margaret.pe...@sdstate.edu> Subject: [Histonet] xylene substitutes To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <fca5ef47f9bc694cbb4c58fea04219634cd0042...@sdsu-mbx.jacks.local> Content-Type: text/plain; charset="us-ascii" We use formula 83 for everything and do not have a problem with a film. We have found that we must use isopropal alcohol instead of reagent alcohol in the final dehydration steps. Margaret Perry SDSU ------------------------------ Message: 3 Date: Wed, 12 May 2010 14:20:36 -0400 From: "Jones, Laura" <lpjo...@srhs-pa.org> Subject: [Histonet] RE: AMT Cover To: "'Goins, Tresa'" <tgo...@mt.gov>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <4ae8039aea096143b965cbc6d0921668022ca80...@exch2007.srhs-pa.org> Content-Type: text/plain; charset="us-ascii" What on earth were they thinking?! -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Goins, Tresa Sent: Wednesday, May 12, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: AMT Cover To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover. Dr. Tresa Goins Department of Livestock Bozeman, MT 59718 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Wed, 12 May 2010 13:21:59 -0500 From: <wanda.sm...@hcahealthcare.com> Subject: [Histonet] RE: AMT Cover To: <tgo...@mt.gov>, <histonet@lists.utsouthwestern.edu> Message-ID: <9e2d36ce2d7cba4a94d9b22e8328a3ba138aa82...@nadcwpmsgcms03.hca.corpad.net> Content-Type: text/plain; charset="us-ascii" We felt offended by the cover also!!!! Even my Cytotechs felt that wasn't necessary!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, May 12, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: AMT Cover To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover. Dr. Tresa Goins Department of Livestock Bozeman, MT 59718 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Wed, 12 May 2010 12:07:51 -0700 From: Jennifer Anderson <jander...@halozyme.com> Subject: [Histonet] RE: AMT Cover To: "'wanda.sm...@hcahealthcare.com'" <wanda.sm...@hcahealthcare.com>, "tgo...@mt.gov" <tgo...@mt.gov>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <5f7cc9b788911848a79bc83453a3d653040c50b...@tomlinson.hti.com> Content-Type: text/plain; charset="iso-8859-1" Greetings to all. I am SO SO happy to see that others felt the same way about the catalog cover. I immediately went to the AMT website and gave them my opinion. They could definitely have choreographed a more sensitive image. I received a very nice email back, apologizing for any offense, of course un-intentional. The advertising and media department head who sent the email assured me that they had positive feedback on the cover, in support of better techniques for cervical cancer screening. I hope they can make better marketing decisions in the future. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) jander...@halozyme.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Wednesday, May 12, 2010 11:22 AM To: tgo...@mt.gov; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: AMT Cover We felt offended by the cover also!!!! Even my Cytotechs felt that wasn't necessary!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, May 12, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: AMT Cover To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover. Dr. Tresa Goins Department of Livestock Bozeman, MT 59718 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. ------------------------------ Message: 6 Date: Wed, 12 May 2010 15:11:19 -0400 From: "Terri Brown" <terri.br...@northside.com> Subject: RE: [Histonet] RE: AMT Cover To: "Jones, Laura" <lpjo...@srhs-pa.org>, "Goins, Tresa" <tgo...@mt.gov>, <histonet@lists.utsouthwestern.edu> Message-ID: <731941c266951a47bef11e5efaaed9c90196e...@nsmvexch01.northside.local> Content-Type: text/plain;charset="us-ascii" How do we see the cover on line? We did not receive a copy. Terri H. Brown,, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.br...@northside.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Wednesday, May 12, 2010 2:21 PM To: 'Goins, Tresa'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: AMT Cover What on earth were they thinking?! -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Goins, Tresa Sent: Wednesday, May 12, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: AMT Cover To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover. Dr. Tresa Goins Department of Livestock Bozeman, MT 59718 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ------------------------------ Message: 7 Date: Wed, 12 May 2010 15:23:59 -0400 From: "Bell, Lynne" <lynne.b...@cvmc.org> Subject: [Histonet] RE: AMT Cover To: "'Goins, Tresa'" <tgo...@mt.gov>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <f9e5562685af9b498d9d8c1ec996d2440f4011b...@cvmc-email.cvmc.org> Content-Type: text/plain; charset="us-ascii" I had this catalog on my desk and everyone that came in to Histology was astounded by the cover. No need for the dorsal lithotomy position! Lynne A. Bell, HT (ASCP) Technical Specialist, Histology Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4923 ------------------------------ Message: 8 Date: Wed, 12 May 2010 12:35:58 -0700 From: "Elliott, Rachel A." <rachel.elli...@thermofisher.com> Subject: [Histonet] RE: AMT Cover To: Jennifer Anderson <jander...@halozyme.com>, "'wanda.sm...@hcahealthcare.com'" <wanda.sm...@hcahealthcare.com>, "tgo...@mt.gov" <tgo...@mt.gov>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <7b380bf8e6354f4994f20e095d43f171ef6c7...@uspho-mxvs01.amer.thermo.com> Content-Type: text/plain; charset="us-ascii" So how can I see this cover? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Wednesday, May 12, 2010 3:08 PM To: 'wanda.sm...@hcahealthcare.com'; tgo...@mt.gov; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: AMT Cover Greetings to all. I am SO SO happy to see that others felt the same way about the catalog cover. I immediately went to the AMT website and gave them my opinion. They could definitely have choreographed a more sensitive image. I received a very nice email back, apologizing for any offense, of course un-intentional. The advertising and media department head who sent the email assured me that they had positive feedback on the cover, in support of better techniques for cervical cancer screening. I hope they can make better marketing decisions in the future. Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11388 Sorrento Valley Road San Diego, CA 92121 858-704-8333 (office) jander...@halozyme.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wanda.sm...@hcahealthcare.com Sent: Wednesday, May 12, 2010 11:22 AM To: tgo...@mt.gov; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: AMT Cover We felt offended by the cover also!!!! Even my Cytotechs felt that wasn't necessary!!! Wanda WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Wednesday, May 12, 2010 12:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: AMT Cover To anyone who has received the Master Tech Flyer Volume XXXI Issue I - please contact Master Tech to let them know what you think of the cover. Dr. Tresa Goins Department of Livestock Bozeman, MT 59718 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 12 May 2010 16:39:04 -0500 From: "Montina Van Meter" <montina.vanme...@pbrc.edu> Subject: [Histonet] 2010 Louisiana Society for Histotechnology State Meeting - Reservation Extension! To: <histonet@lists.utsouthwestern.edu> Message-ID: <4fe7fb862e90e448ae32388e759220e502507...@pbrcas31.pbrc.edu> Content-Type: text/plain; charset="us-ascii" Hello Histonetters, The Louisiana Society for Histotechnology would like to invite you to our annual Symposium/Conference on June 4 & 5, 2010, in Baton Rouge, LA. Meeting location: The Embassy Suites Hotel 414 Constitution Ave. Baton Rouge, LA 70808 We have a block of rooms reserved for attendees at the special rate of $99.00. The cut-off date for reservations has been extended to May 20, 2010. Following that date the rooms may be reserved at that rate per availability. The hotel will honor this rate two days prior and two days after our meeting (please contact the Embassy Suites Hotel for further information: 1-225-924-6566 or 1-800-Embassy). A complimentary hotel shuttle service is provided for those flying into Baton Rouge. Great dining, beautiful southern plantations and gambling boats on the Mississippi River are just a few of the attractions in the Baton Rouge area. Remember to ask for the Louisiana Society for Histotechnology group when placing your reservation. Walk-ins are always welcome! If you have any questions please contact: Tina Van Meter at 225-603-0953 or vanme...@pbrc.edu. Workshops: WS #1: FISH - IT'S NOT JUST FOR DINNER! Bonnie Whitaker, HT (ASCP) - sponsored by Cell Marque OSU Medical Center WS #2: What is the Tumor Registry? Cynthia Boudreaux, LPN, CTR Touro Infirmary WS #3: Veterinary vs. Clinical - Which Career is Best for Me? Pam Marcum, B.S., M.S., HT, (ASCP) UAMS WS #4: Microtomy, It's About Technique! Mari Ann Mailhiot, BA, HT (ASCP) Leica Microsystems WS #5: Boot Camp for Histotechs Mari Ann Mailhiot, BS, HT (ASCP) Leica Microsystems WS #6: So You Want to Know More About Things Your Lab Doesn't Do Pam Marcum, B.S., M.S., HT (ASCP), UAMS Bonnie Whitaker, HT (ASCP) QIHC OSU Medical Center WS #7: Dilution, Titrations, and Good Pipetting in the IHC Laboratory Robin Simpkins, HT (ASCP) Biocare Medical WS #8: Detection Chemistry & Antibody Production Tania Ewing-Finchem, HT (ASCP) Ventana Roche Hope to see you in June! Tina LSH Secretary ------------------------------ Message: 10 Date: Wed, 12 May 2010 23:54:55 +0200 From: "V. Neubert" <histonet.nos...@vneubert.com> Subject: Re: [Histonet] Xylene substitutes To: histonet@lists.utsouthwestern.edu Message-ID: <4beb23af.3080...@vneubert.com> Content-Type: text/plain; charset=ISO-8859-1 My hand staining setup contained some commercial naphta mix, which is comparable to any other petroleum benzine solvant, for dewaxing. For dehydrating/coverslipping, I used "n-Butyl acetate" a.k.a. "butyl ethanoate". Coverslipping can be done with any xylene soluble glue. > What are labs using for xylene substitute. I will be using it in a hand > staining setup for Mohs. Feed back on Histo-Clear, Clear solve, S-3 > and formular 83. > > Thanks in advance. > > Dawn Oakes HT > > > > > ------------------------------------------------------------------------- > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended individual(s) named above and may contain > confidential, privileged, and/or protected information. Any unauthorized > review, use, disclosure, copying, or distribution of its contents is > prohibited. If you are not the intended recipient, you have received this > email in error. If so, please notify the sender immediately by reply email > and delete/destroy the original and all copies of this communication. Also > know that Internet e-mail is not secure. In choosing to communicate with > Olympic Medical Center by email you will assume these confidentiality risks. > Internet messages may become corrupted, incomplete, or may incorrectly > identify the sender. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 11 Date: Wed, 12 May 2010 14:57:36 -0700 (PDT) From: Cristi stephenson <cls71...@sbcglobal.net> Subject: [Histonet] Gastric biopsies To: histonet@lists.utsouthwestern.edu Message-ID: <304545.34701...@web81204.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello Histoland, I was wondering if anyone out there does stains other than H&E 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi ------------------------------ Message: 12 Date: Thu, 13 May 2010 08:49:31 +1000 From: "Tony Henwood" <antho...@chw.edu.au> Subject: RE: [Histonet] Forwarding Request for Control Mehtod To: "Ian Montgomery" <ian.montgom...@bio.gla.ac.uk>, <histonet@lists.utsouthwestern.edu> Message-ID: <ae22dcd58fa1b045b26ff61d7259578f1cb...@x2k3.nch.kids> Content-Type: text/plain; charset="us-ascii" Ah yes, I remember, actually I don't. It was a bit of a blur!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Wednesday, 12 May 2010 9:32 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Forwarding Request for Control Mehtod Tony, Is this a universal teenage thing, filthy bedrooms? I'm convinced there are alien life forms in my daughter's bedroom. They make the strangest noises, grunts and groan and "I'm bored." Me, I was the perfect teenager apart from the Celtic thing of taking to the drink and of course, lusting after girls. Ian. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Henwood Sent: 12 May 2010 01:54 To: WILLIAM DESALVO; histonet Subject: RE: [Histonet] Forwarding Request for Control Mehtod Ian, What I have done in the past is to raid the Staff Fridge (or your son's bedroom!). There is usually old food left there that has gone moldy - bread or even strawberries. Wearing gloves & in a fume hood slice the bread or fruit into slices (ensuring visible growth of fungi is present), fix for two or more days in NBF, process as usual. Voila - fungi controls Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO Sent: Wednesday, 12 May 2010 4:01 AM To: histonet Subject: [Histonet] Forwarding Request for Control Mehtod > I wonder if you could help me find someone who would kindly donate a > paraffin tissue block containing candida or other fungi to be used as > a control block. We have had difficulty in obtaining such tissue and > have tried all the hospitals in northern Ireland for a tissue block > suitable enough to act as a control. Many of the pathology departments > are in the same position as ourselves in that the control tissue being > used is getting in short supply. > > I tried to make one using a culture from microbiology with fresh lambs > lung from a local abattoir but unfortunately the tissue seems to stain > with PAS as well and the candida where obscured although visible > probably due to post mortem changes in the tissue. > Alternatively have any of your members successfully produced fungal > control material by other means. > > Thanking you in advance > Ian Clarke > Biomedical Scientist > Cellular Pathology Department > Craigavon Area Hospital > Sothern Health and Social Services Trust. > 66 Lurgan Road > Craigavon > BT63 5QQ > Northern Ireland William DeSalvo, B.S., HTL(ASCP) _________________________________________________________________ The New Busy is not the too busy. Combine all your e-mail accounts with Hotmail. http://www.windowslive.com/campaign/thenewbusy?tile=multiaccount&ocid=PI D28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_4___________________________ ____________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ **** ***** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ************************************************************************ **** ***** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Wed, 12 May 2010 19:46:32 -0400 From: Brandi Higgins <brandihigg...@gmail.com> Subject: Re: [Histonet] Gastric biopsies To: Cristi stephenson <cls71...@sbcglobal.net> Cc: histonet@lists.utsouthwestern.edu Message-ID: <aanlktimd_ff5nafuhkpbth-f4kvybnbquighzrgto...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 We do Diff Quik on all gastric biopsies, and we do Alcian Blue on all esophageal biopsies with Barrett's indicated by the GI Dr. Our other special stains are by request, but we almost always end up doing retic and trichrome on liver bx submitted with hepatitis as Clinical Dx. Hope this helps. Brandi Higgins BS, HT(ASCP) On Wed, May 12, 2010 at 5:57 PM, Cristi stephenson <cls71...@sbcglobal.net>wrote: > Hello Histoland, > I was wondering if anyone out there does stains other than H&E 's on > specific tissues. For example, does anyone run stains to rule out H. > pylori on all gastric biopsies? Any information as to the standards in > other labs would be great. > Thanks, > Cristi > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 14 Date: Wed, 12 May 2010 21:57:10 -0400 (EDT) From: susanfpl...@aim.com Subject: Re: [Histonet] Gastric biopsies To: cls71...@sbcglobal.net, histonet@lists.utsouthwestern.edu Message-ID: <8ccc056bbca5837-89c-3...@webmail-d041.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies. Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori. We occasionally did H. Pylori using immunohistochemistry. We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies. Hope this helps. Susan Plaza, HT (ASCP), QIHC -----Original Message----- From: Cristi stephenson <cls71...@sbcglobal.net> To: histonet <histonet@lists.utsouthwestern.edu> Sent: Wed, May 12, 2010 5:25 pm Subject: [Histonet] Gastric biopsies Hello Histoland, was wondering if anyone out there does stains other than H&E 's on specific issues. For example, does anyone run stains to rule out H. pylori on all astric biopsies? Any information as to the standards in other labs would be reat. hanks, risti ______________________________________________ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 12 May 2010 19:18:10 -0700 From: "Michelle MacVeigh-Aloni" <macve...@usc.edu> Subject: [Histonet] How do I subscribe? To: <Histonet@lists.utsouthwestern.edu> Message-ID: <ad1f39a8a74644308b3a7d89f0b80...@dfs66dd1> Content-Type: text/plain; charset="iso-8859-1" Hi all, Can someone tell me - how does one subscribe to Master Tech Flyer? Thank you in advance Michelle ------------------------------ Message: 16 Date: Wed, 12 May 2010 22:38:10 -0400 From: <dcoj...@tampabay.rr.com> Subject: [Histonet] job opening for cytology/ FISH and histology supervisor - Florida To: "'histonet'" <Histonet@lists.utsouthwestern.edu> Message-ID: <!~!uenerkvcmdkaaqacaaaaaaaaaaaaaaaaabgaaaaaaaaadq7zshuc4ki+f530qnqczmkaaaaqaaaau8qpj2e83e+tdvjs6kiw8qeaa...@tampabay.rr.com> Content-Type: text/plain; charset="us-ascii" I have a job opening for a cytotechnologist (non-gyn only) preferably with experience with Urovysion/FISH (not required but willing to learn) in the Tampa bay area. I also know of a job opening for a histologist; supervisory position (part time or full time) in Ormond Beach, Florida Please call Diane anytime for details 813-843-0483 ------------------------------ Message: 17 Date: Thu, 13 May 2010 08:05:57 -0400 From: "Gill, Caula A." <cg...@marylandgeneral.org> Subject: RE: [Histonet] Gastric biopsies To: "Cristi stephenson" <cls71...@sbcglobal.net>, <histonet@lists.utsouthwestern.edu> Message-ID: <087a9911bbafde4b8151cb148586e2c23a9...@mdgen-exch1.marylandgeneral.org> Content-Type: text/plain; charset="iso-8859-1" Hello Cristi, We routinely do a steiner on all Gastric Bx.'s we also do Pas W and WO digestion, Fe, Trichrome and retic on all liver with DX of Hepatitis. GMS-P on Lung bx POS. for HIV if requested by Dr. Elastic, retic and Trichrome on Temporal Arteries. Caula Gill (HT) ASCP -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson Sent: Wednesday, May 12, 2010 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gastric biopsies Hello Histoland, I was wondering if anyone out there does stains other than H&E 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Thu, 13 May 2010 05:09:34 -0700 From: "Heckford, Karen - SMMC-SF" <karen.heckf...@chw.edu> Subject: RE: [Histonet] Are Histotechs considered "exempt" employees? To: "joelle weaver" <joellewea...@hotmail.com> Cc: histonet@lists.utsouthwestern.edu Message-ID: <2842dc75ae43aa4b92954cfb31781bc105697...@chw-msg-301.chw.edu> Content-Type: text/plain; charset="US-ASCII" It's just crazy the wage differences around the country. But we should fight for the higher wages in whatever location we live in. Not everyone can do this type of job and be good at it. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 ________________________________ From: joelle weaver [mailto:joellewea...@hotmail.com] Sent: Thursday, May 13, 2010 4:24 AM To: Heckford, Karen - SMMC-SF; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] Are Histotechs considered "exempt" employees? Boy, I wish! I live in Ohio with over 10 years bench experience, supervisory experience, HTL and almost a masters (4 classes left)- and I don't approach the HT scale you proposed-even the minimum. > Date: Tue, 11 May 2010 05:15:40 -0700 > From: karen.heckf...@chw.edu > To: sandoval.1...@hotmail.com > Subject: RE: [Histonet] Are Histotechs considered "exempt" employees? > CC: histonet@lists.utsouthwestern.edu > > $16.15 per hour. They are getting a great deal. I believe you should > be making at least double that. What I believe is happening is that a > lot of offices are now starting to open their own labs and they are > trying to get us cheap. We all need to stick to our guns and not let > them get us cheaper than we are worth. Yes, I realize that San > Francisco is more but the starting wage for a HT's should be $25.00 to > $30.00 an hour to start for a newly certified tech. No matter of > location in California. You can always negotiate higher if you have > more experience and more certifications. > > Just my two cents worth, > > Karen Heckford HT ASCP CE > Lead Histology Technician > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Anthony > Sandoval > Sent: Monday, May 10, 2010 9:01 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Are Histotechs considered "exempt" employees? > > Hello fellow Histotechs, I have recently become certified as an HTL and > was > wondering if anyone out there is an 'exempt' employee? I live in > California > and feel that I am being taken advantage of. I make 16.15$ per hour and > > frequently work 50 hour weeks. Am I off base? should I just be grateful > that > I have a job, as my employer so frequently reminds me? Thank you > Histonet! > you have been an invaluable resource in my career and assisting me in > passing the HTL! > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. Learn more. <http://www.windowslive.com/campaign/thenewbusy?ocid=PID28326::T:WLMTAGL :ON:WL:en-US:WM_HMP:042010_1> ------------------------------ Message: 19 Date: Thu, 13 May 2010 08:37:25 -0400 From: "Cynthia Pyse" <cp...@x-celllab.com> Subject: RE: [Histonet] Gastric biopsies To: "'Cristi stephenson'" <cls71...@sbcglobal.net>, <histonet@lists.utsouthwestern.edu> Message-ID: <000001caf299$0ad3a100$207ae3...@com> Content-Type: text/plain; charset="iso-8859-1" Christi We run a diff stain on all gastric bx suspected of H. pylori as routine, it saves us time. Cindy Pyse, CLT, HT (ASCP) Histology Supervisor X-Cell Laboratories e-mail cp...@x-celllab.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson Sent: Wednesday, May 12, 2010 5:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gastric biopsies Hello Histoland, I was wondering if anyone out there does stains other than H&E 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Thu, 13 May 2010 06:10:22 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Gastric biopsies To: histonet@lists.utsouthwestern.edu, Cristi stephenson <cls71...@sbcglobal.net> Message-ID: <120364.96574...@web65704.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 For gastric biopsies we routinely did modified Steiner for H. pylori René J. --- On Wed, 5/12/10, Cristi stephenson <cls71...@sbcglobal.net> wrote: From: Cristi stephenson <cls71...@sbcglobal.net> Subject: [Histonet] Gastric biopsies To: histonet@lists.utsouthwestern.edu Date: Wednesday, May 12, 2010, 5:57 PM Hello Histoland, I was wondering if anyone out there does stains other than H&E 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Thu, 13 May 2010 09:37:44 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: RE: [Histonet] Gastric biopsies To: "susanfpl...@aim.com" <susanfpl...@aim.com>, "cls71...@sbcglobal.net" <cls71...@sbcglobal.net>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e164015de06...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" For H. pylori we do a Genta, which is a modified Steiner, with Alcian blue and H & E over it. I've finally gotten the docs to wait to make sure they need specials on everything else, so as not to waste. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of susanfpl...@aim.com Sent: Wednesday, May 12, 2010 21:57 To: cls71...@sbcglobal.net; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gastric biopsies We routinely do PAS with and without digestion, Trichrome and Iron on liver biopsies. Some doctors I've worked with preferred an Alcian Yellow/Toluidine Blue or a Giemsa for H. Pylori. We occasionally did H. Pylori using immunohistochemistry. We also do Iron stains on all bone marrow smears (fixed in methanol) and biopsies. Hope this helps. Susan Plaza, HT (ASCP), QIHC -----Original Message----- From: Cristi stephenson <cls71...@sbcglobal.net> To: histonet <histonet@lists.utsouthwestern.edu> Sent: Wed, May 12, 2010 5:25 pm Subject: [Histonet] Gastric biopsies Hello Histoland, was wondering if anyone out there does stains other than H&E 's on specific issues. For example, does anyone run stains to rule out H. pylori on all astric biopsies? Any information as to the standards in other labs would be reat. hanks, risti ______________________________________________ istonet mailing list isto...@lists.utsouthwestern.edu ttp://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 22 Date: Thu, 13 May 2010 09:44:33 -0400 From: Joyce Cline <joyce.cl...@wchsys.org> Subject: RE: [Histonet] Gastric biopsies To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <fe2197d76184f4408cfebd95c28282685bf1e1d...@wchsxchcm.wchsys.org> Content-Type: text/plain; charset="us-ascii" We do Iron,Trichrome, Pas with and without Diastase and Retic on liver biopsies if requested by the grossing pathologist. We do Diff-Quik on all stomach biopsies, and if requested by the pathologist an Hp immno. A tricchrome is done on colon with a diagnosis of microscopic or collagenous colitis . A pas light green is done on esophogeal biopsies for candida. Any other specials are done at the request of the pathologist. Joyce Cline, Technical Specialist Hagerstown Medical Laboratory 301-665-4980 fax 301-665-4941 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi stephenson [cls71...@sbcglobal.net] Sent: Wednesday, May 12, 2010 5:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gastric biopsies Hello Histoland, I was wondering if anyone out there does stains other than H&E 's on specific tissues. For example, does anyone run stains to rule out H. pylori on all gastric biopsies? Any information as to the standards in other labs would be great. Thanks, Cristi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ Message: 23 Date: Thu, 13 May 2010 09:59:18 -0400 From: "Marquisha Paul" <mp...@alltech.com> Subject: [Histonet] Tilapia Fish Eye To: <histonet@lists.utsouthwestern.edu> Message-ID: <421ee4fdc90a744cb1ddddd21a1bc56e09fbb...@ergo.alltech-bio.com> Content-Type: text/plain; charset="iso-8859-1" Dear Histonetters, Does anyone out there have experience with processing whole tilapia fish eye? We formalin fixed our specimen, dehydrated with EtOH and cleared with Citrisolv before embedding in paraffin wax. At the microtome we encountered all kinds of chatter. Any advice is greatly appreciated. Thanks, Marquisha Paul ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 78, Issue 17 **************************************** mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet