Dear colleagues,
has anyone experience with embedding and sectioning of tissue attached to silicone, e.g. PDMS? I tried cryosectioning as well as sectioning of paraffin containing thin sheets of silicone with-or without tissue. Both methods were not very encouraging. The silicone seems to be able to 'move' within the block and gets eventually disrupted after/while touching the blade. I guess, because of the missing stable connection between the silicone and the embedding medium. Another problem is swelling of the polymer in the organic solvent-but, I can deal with that. Any help will be greatly appreciated. Thanks, Klaus Klaus Kruttwig INM-Leibniz Institute for New Materials Saarbruecken, Germanz -------------------------------------------------------------------------------- INM - Leibniz-Institut für Neue Materialien gGmbH Campus D2 2, 66123 Saarbrücken Homepage: www.leibniz-inm.de<http://www.inm-gmbh.de/> Sitz der Gesellschaft: Saarbrücken Rechtsform: gGmbH Amtsgericht Saarbrücken, HRB 8525 Geschäftsführer: Prof. Dr. Eduard Arzt (Vorsitz), Prof. Dr. Aránzazu del Campo, Günter Weber Kuratoriumsvorsitzende: Dr. Susanne Reichrath USt.-ID: DE 138167776 -------------------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet