[Histonet] in-situ hybridization

2011-04-19 Thread Bales, Candy A
This questions is for the labs that do in-situ hybridization for diagnostic purposes. Do you take slides for ISH from other labs and if so, please contact me via my email. My next question is; one of my doc's is interested in us doing ISH herein our lab but I have been putting her off with the

Re: [Histonet] in-situ hybridization

2011-04-19 Thread Emily Sours
Roche Diagnostics has great DIG labels, antibodies and color reaction chemicals. It is a pain to just start up, you need to buy a lot of stuff for it. Could you use someone else's set up and pay for part of the chemicals? Emily A great book should leave you with many experiences, and slightly

[Histonet] in situ hybridization

2011-01-20 Thread louise renton
Hi all, after a decade of not doing ISH, I have been asked to initiate a project in our department. needless to say i am VERY rusty. can any one suggest: - a good on-line resource to brush up my knowledge - a company that have reference materials/handbooks etc (I seem to recall Roche

[Histonet] in situ hybridization histochemistry question about radioisotope

2010-03-13 Thread Susan Bachus
I have routinely seen that we obtain slightly different results (like 10-20% variance) in using 35S dATP for TdT-catalyzed tailing reactions of oligonucleotides from month to month as we use different batches (made fresh monthly) of the 35S dATP. But on a few occasions over the years we have