Re: [Histonet] malarial pigment

2017-03-07 Thread Paula Keene Pierce via Histonet
Lee Luna's third edition. Page 43, Method II 1. Deparaffinize and hydrate to water.2. Wash in distilled water.3. Immerse slides in 100 ml of 70% alcohol, to which has been added 2 ml of 28% ammonium hydroxide, for 3 hours.4. Rinse in water.5. Rinse in 1% glacial acetic acid.6. Wash well in

[Histonet] malarial pigment

2017-03-07 Thread Sanders, Jeanine (CDC/OID/NCEZID) via Histonet
Good morning! Does anyone know of a method using ammonium hydroxide to remove malarial pigment from tissue sections? Thanks! Jeanine H. Sanders Infectious Diseases Pathology Branch Centers for Disease Control and Prevention 1600 Clifton Rd., NE MS-G32 Atlanta, GA 30329