Ruegg [mailto:pru...@ihctech.net]
Sent: Tuesday, November 01, 2011 1:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] mast cells for arteries
Does anyone have a go to stain for Mast Cells besides Tol Blue? We are
trying to demonstrate the presence of mast cells in human arteries ffpe
If you don't want the with purple-red metachromasia, try neutral red, which
gives an orange-red metachromasia and true red nuclei.
John Kiernan
Anatomy, UWO
London, Canada
= = =
On 01/11/11, Patsy Ruegg pru...@ihctech.net wrote:
Does anyone have a go to stain for Mast Cells besides Tol
] mast cells for arteries
Does anyone have a go to stain for Mast Cells besides Tol Blue? We are
trying to demonstrate the presence of mast cells in human arteries ffpe.
Also, what would be a good positive control to use for mast cell staining,
we used NH Skin and got maybe a few mast cells
Does anyone have a go to stain for Mast Cells besides Tol Blue? We are
trying to demonstrate the presence of mast cells in human arteries ffpe.
Also, what would be a good positive control to use for mast cell staining,
we used NH Skin and got maybe a few mast cells but I would like to use
Go to http://stainsfile.info/StainsFile/stain/cell/aldtolblue.htm for a
reliable method. It still uses toluidine blue, but the staining is not
based on metachromasia and mast cell granules are dark blue. Most
toluidine blue samples will work, but occasionally one doesn't. Fisher
brand is OK as
A good one that I used to use was the Naphthol AS-D Chloroacetate-esterase
which stains the non specific esterase activity in mast cells. It is a
pain to run, but it is one of the most beautiful. A bright red mast cell
against a pale background. We got it from Vacca, p 530-533.
On Tue, Nov 1,
