Good morning Rene,
I have worked out special staining IHC protocols to work on celloidin cut
sections from 60um to 600um thick. These are
free-floating human whole brainstem & whole brain sections. The IHC is amazing
using single, double & triple. We save on
antibodies, ABC, polymer, TSA
You have a special project → special tasks so your approach has to be equally
special.Large brain sections are usually stained while floating but for IH with
different and successive steps requiring very expensive reagents, floating
sections is not well suited.You should affix the sections to
Or lab is currently processing a human whole brain. In about a month or two,
the whole brain, which will be encased
in celloidin & serial sections will be cut at 120um each. Now, we’ve bought an
old Tetrander cast iron microtome. If
you haven’t seen one of these microtomes, I can tell you
