Hi all,
Bad day here. Left the IHC stainer to attend a meeting and the volume of the
DAB was not sufficient to stain all slides. By the time I realized this, they
had been counterstained. I immediately applied DAB to the affected slides but
naturally the antigen is not abundant if present
Oops. Yes hematoxylin staining interferes with DAB staining. Sorry! You can
save the slides by re-retrieving and restaining.
Will
Sent from my iPhone
On Oct 27, 2011, at 10:59 AM, Denise G Crowley dencr...@mit.edu wrote:
Hi all,
Bad day here. Left the IHC stainer to attend a meeting
www.ihctech.net
www.ihcrg.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William
Sent: Thursday, October 27, 2011 12:03 PM
To: Denise G Crowley
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] oops
That was backwards =)
Don't stain mouse cells...stain human ones
Sarah Goebel-Dysart, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
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