I am having trouble posting to the histonet for some reason. I am looking for
condition and incubation times for p16 on the Bond. Our new pathologist says
our p16 looks overstained to him and not at all what he is accustomed to seeing
and I am trying to get the staining more to his liking,
We dilute the CINtec p16 RTU antibody (Ventana/Roche) 1:10 (15' primary
antibody incubation) and use high pH retrieval (H2) for 10' on the Bond Max.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic
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If anyone out there is performing p16 on the Leica Bond immunostainer, could
you please tell me where you purchase your antibody and what protocol you are
using?
Thanks so much!
Kathy Maddox HT{ASCP}
Lake Charles, Louisiana
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When we obtained the p16 antibody from mtm Laboratories we used it a dilution
of 1:10 on the Bond Max. I hope that the concentration will not change now that
Ventana is selling it.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Assistant Director, Anatomic Pathology
