For another method that works on Ventana XTs and BenchMarks: BD antibody (cat.#: 550834), 1:10, mild CC1, 32 " incubation @ 42 degrees, Hem II/8"
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Turner Sent: Thursday, February 24, 2011 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Several of you have asked that I share our protocol for the P16 on the Ventana XT. I apologize for the delay, but here is what we have worked up and it performs well in our lab: CC1 Standard Antibody incubation @ 37 degrees C. for 28 minutes Amplification Hematoxylin counterstain 4 minutes Bluing Reagent 4 minutes We place the commercial antibody from the CINtec kit in a prep kit and use it as an RTU. Mark A. Turner, Ph.D. HT (ASCP) QIHC | Test Development Team Lead | Department of Laboratory Medicine and Pathology | 480-301-8026 | Fax: 480-301-8372 | turner.m...@mayo.edu Mayo Clinic in Arizona | 13400 E. Shea Blvd. | Scottsdale, AZ 85259 | www.mayoclinic.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet