...
2009-03-06
TF
Geoff McAuliffe
? 2009-03-06 23:37:34
shymaa shawadfy
??? histonet
??? Re: [Histonet] postnatal brain sections using vibratome
If you put a little glutaraldehyde (0.25%) in the fixaitve you perfuse
with but not in the post fix solution the brain will be
Hi, I dont think glutaraldehyde is good for many antigens...though the brain
does become firmer...
2009-03-06
TF
发件人： Geoff McAuliffe
发送时间： 2009-03-06 23:37:34
收件人： shymaa shawadfy
抄送： histonet
主题： Re: [Histonet] postnatal brain sections using vibratome
If you put a little
If you put a little glutaraldehyde (0.25%) in the fixaitve you perfuse
with but not in the post fix solution the brain will be noticably firmer
and your antigen may survive.
Good luck!
Geoff
shymaa shawadfy wrote:
Dear all
I am trying to use vibratome 50 µm thick sections for immunofluoresc
Yes, definitely make sure to do the 20% sucrose overnight, even try 30%, or
grades going from 10%-20%-30%, switching to the next higher when the brain
becomes saturated enough to fall to the bottom of the container. We did
this in a previous lab with rat brains and it worked well.
Merced
--O
2009-03-06
TF
发件人： shymaa shawadfy
发送时间： 2009-03-06 10:55:20
收件人： histonet@lists.utsouthwestern.edu
抄送：
主题： [Histonet] postnatal brain sections using vibratome
Dear all
I am trying to use vibratome 50 祄 thick sections for immunofluorescence
using Postnatal day 0 brains. The probl
Dear all
I am trying to use vibratome 50 µm thick sections for immunofluorescence
using Postnatal day 0 brains. The problem is that brains are very soft and
are usually destroyed upon handling and the agarose is separated form the
brain.
My used protocol was: perfusion with 4 % PFA for 3 min, fol
