Re: [Histonet] pouring of liquid nitrogen into a cryostat

2011-10-17 Thread Emily Sours
I would suggest dry ice (on top of something plastic on top of the metal shelves), NOT liquid nitrogen. Dry ice is usually cold enough to bring our cryostat to -28 C when it won't cool down past -24 C because of frost on the compressor. I don't think it actually makes the cryostat colder than tha

Re: [Histonet] pouring of liquid nitrogen into a cryostat

2011-10-16 Thread Tony Reilly
Hi Louise Not a very safe practice. I have heard of people using a bag of dry ice pellets rested against the knife holder to lower the temperature of the knife, both for when the chamber is too warm or the tissue needs to be cut at a lower temperature, such as fat. regards Tony Tony

Re: [Histonet] pouring of liquid nitrogen into a cryostat

2011-10-14 Thread Rene J Buesa
Never heard of that and I would not do it at all. Just leave the cryostat to freeze itself. René J. --- On Fri, 10/14/11, Kaye Ryan wrote: From: Kaye Ryan Subject: [Histonet] pouring of liquid nitrogen into a cryostat To: histonet@lists.utsouthwestern.edu Date: Friday, October 14, 2011, 12

Re: [Histonet] pouring of liquid nitrogen into a cryostat

2011-10-14 Thread Louise Renton
bad idea - what about splash ups from the chamber? Very scary to contemplate On Fri, Oct 14, 2011 at 6:36 PM, Kaye Ryan wrote: > Hi Everyone, > > Has anyone ever heard of pouring liquid nitrogen into the chamber of a > cryostat that has warmed up to help cool it down more quickly? Does > this d

[Histonet] pouring of liquid nitrogen into a cryostat

2011-10-14 Thread Kaye Ryan
Hi Everyone, Has anyone ever heard of pouring liquid nitrogen into the chamber of a cryostat that has warmed up to help cool it down more quickly? Does this do harm to the chamber or mechanisms of the cryostat? Any information would be appreciated. Thanks ___