RE: [Histonet] processor stopped - help!

2011-10-24 Thread Daniela Bodemer
I am glad to hear the samples will be fine. Thanks everyone! Daniela Bodemer Research Assistant Surgical Research, Infection and Immunity Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Victoria 3052 Australia T 03 9345 5930 T (03

Re: [Histonet] processor stopped - help!

2011-10-24 Thread William Chappell
Yes, nothing to worry about here (I am assuming an autotechnicon?). The first paraffin would have dried and sealed the blocks keeping the tissue just fine, however you are probably right in thinking they did not get enough paraffin infiltration. Try another hour in the second paraffin. If you

[Histonet] processor stopped - help!

2011-10-24 Thread Daniela Bodemer
Hi all, When I arrived the lab this morning I found the processor basket with my cassettes stuck between the two paraffin tanks. I suppose it went through the first cycle but not the second one, so its missing 1.5 hrs of paraffin infusion. Should I put them back in hot paraffin for 1.5 hours or