Hi Everyone, Does anyone know how close in proximity 2 proteins need to be for them to show up as co-localized via immunofluorescence staining? I am trying to compare routine 2-color IF vs. the Proximity Ligation Assay (which will not show co-localization unless the proteins are withing 40 nM of each other). Thanks, Kim
Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet