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> histonet-requ...@lists.utsouthwestern.edu
> CC:
> Subject: [Histonet] Quenching auto fluorescence in human tissue
>
> Hi all,
>
>
>
> I have been experiencing trouble with my secondary antibody when doing
> my immuno with human colon tissue.
>
> I use to use FITC 48
Hi all,
I have been experiencing trouble with my secondary antibody when doing
my immuno with human colon tissue.
I use to use FITC 488 as my secondary antibody and changed over to
Alexa488 due to the FITC's manufacturing stop.
The auto fluorescence of the human tissue is quenched with Chicag
Good morning.
I'm wondering how to quench auto-fluorescence in tissue from a mouse
that was treated with a FITC-labeled protein. What do people usually
use to quench the endogenous auto-fluorescence, and will this treatment
quench the FITC signal from the labeled protein?
Thanks a lot for your h
