thanks, i contact the author, according to Dr.Atsushi Miyawaki, there
should be no salt in the scaLe solution for clearing of tissues. so the
urea is dissolved in miliQ pure water and adjustment of pH is not
necessary, i guess, for urea.
2011/11/19 John Kiernan jkier...@uwo.ca
The solution
Well, I have used all of the usual suspects over many years but, this one has
taken me by complete surprise!
If anyone can explain the rationale of the reaction, I would be most interested.
One for J Kiernan, I think?
Ah, yes: Sca/e: a chemical approach for fluorescence imaging and
Did anyone know what is the buffer system for the 4M urea? it is important
to adjust and maintain pH to 7.7 according to the paper:
Scale: a chemical approach for fluorescence imaging and reconstruction of
transparent mouse brain
2011/11/18 aget liang agetli...@gmail.com
Hi, anyone has