Re: [Histonet] Re: clearing of whole tissue

2011-11-19 Thread aget liang
thanks, i contact the author, according to Dr.Atsushi Miyawaki, there should be no salt in the scaLe solution for clearing of tissues. so the urea is dissolved in miliQ pure water and adjustment of pH is not necessary, i guess, for urea. 2011/11/19 John Kiernan jkier...@uwo.ca The solution

[Histonet] re: clearing of whole tissue

2011-11-18 Thread Hobbs, Carl
Well, I have used all of the usual suspects over many years but, this one has taken me by complete surprise! If anyone can explain the rationale of the reaction, I would be most interested. One for J Kiernan, I think? Ah, yes: Sca/e: a chemical approach for fluorescence imaging and

[Histonet] Re: clearing of whole tissue

2011-11-18 Thread aget liang
Did anyone know what is the buffer system for the 4M urea? it is important to adjust and maintain pH to 7.7 according to the paper: Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain 2011/11/18 aget liang agetli...@gmail.com Hi, anyone has