Weihua,
I prefer iron gallein elastin stain because it is = very simple to
make and use and gives good contrast compared with some of t= he other
techniques. The recipes and protocol can be found in Humanson's An imal
Tissue Techniques (in addition to many other histological
techniques t= exts), but I can also supply my protocol if need be
(please email and I wil= l send an attachment). Elastic fibers stain
black with gallein, nucle= i pick up some of the stain and will be
dark blue-black. Differentiat= ion is done with ferric chloride and
the counterstain is your personal choi= ce (although acid fuchsin in
saturated picric acid is typically used with g= ood results). The
gallein itself is hardest to find, but can currently be p= urchased
from Fisher Scientific, Spectrum Chemical, or ArtChemicals.com.&nb sp;
I believe that spectrum has it cheapest, and I would buy at least
25g i= f you can because it sometimes goes off the market.
Sincerament= e,
Matt Close
-------= --------------------------
Matthew T. Close
Lehigh University
Department of Biological Sciences
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