This is a resend of a previous message that seems to have been chopped to pieces in cyberspace.
Hope this one comes through better: I prefer to use iron gallein elastin stain for demonstrating elasti fibers. It is simple to make up and gives good contrast compared to some of the other stains. Nuclei stain dark blue, elastin fibers black, everything else is pink or light purple. The protocol can be found in Humanson's Animal tissue techniques and the original article is by Churukian and Schenk (Stain Tech., 1976). I can provide a protocol in .doc format I can send, along with any notes, if you don't have access to either source. Current suppliers of gallein are Fisher Scientific, Spectrum Chemicals, and ArtChemicals.com. Spectrum has it cheapest. Good luck. -Matt --------------------------------- Matthew T. Close Lehigh University Department of Biological Sciences _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet