Robin, I typically stain first with Von Kossa and then counter with MacNeal's.
This provides a very nice contrast where obviously mature mineralized bone is
black and newly formed bone (osteoid) is grayish-green color. Additionally,
your marrow space is nicely contrasted with clear
Does anyone know of a good stain to use to clearly show new bone growth
other than von Kossa stain?
Would appreciate any suggestions anyone might have.
Thank you,
Robin
Robin R. Dean, Ph.D.
Senior Scientist Study Director
Comparative Biosciences, Inc.
786 Lucerne Dr.
Sunnyvale, CA
for visualizing new bone growth
Does anyone know of a good stain to use to clearly show new bone growth
other than von Kossa stain?
Would appreciate any suggestions anyone might have.
Thank you,
Robin
Robin R. Dean, Ph.D.
Senior Scientist Study Director
Comparative Biosciences, Inc.
786 Lucerne Dr
You could do a modified tetrachrome stain, this distinguishes newly woven bone.
On 2/1/11 2:44 PM, Robin Dean robin_d...@compbio.com wrote:
Does anyone know of a good stain to use to clearly show new bone growth
other than von Kossa stain?
Would appreciate any suggestions anyone might have.
You could use May Grunwald-Giemsa modified for tissue (Maximov's procedure).
René J.
--- On Tue, 2/1/11, Vanessa J. Phelan vjp2...@columbia.edu wrote:
From: Vanessa J. Phelan vjp2...@columbia.edu
Subject: Re: [Histonet] stains for visualizing new bone growth
To: Robin Dean robin_d
