I cut 40-50 micron paraffin sections with no difficulty. You may try to use softer paraffin (lower melting temp) or adjust the blade angel. It seems like you have chatter due to hardness of the block or not appropriate blade angel. If tissue is over fixed or overprosessed and hence harder that it should be the thicker you cut the more difficult it will be.
Mesruh Turkekul turke...@mskcc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
