Trichrome: Our Masson Trichrome procedure uses a combined
phosphotungstic/phosphomolybdic acid step for 20 minutes @ RT without
a rinse after, our smooth muscle stays RED..
Bouin's: Working in a rat research lab for years, we fixed all tissue
in bouins for 24 hours, rinsed in water for about an hour and stored
in 70% alcohol till processing. Is all your tissue post fixed in
bouins or just selected items? Why not Bouin's as a primary fixative?
We got great results on PTAH, Trichrome, Luxol Fast Blue stains.
Frozens: CAP says monitor FS times, if 90% are not within 20 minutes,
document the evaluation of why. ANP.11820
Instrument cleaning: We use TBQ which is virucidal and does not rust
instruments like bleach does.
Mast cells: We have not had unintentional mast cell staining on our
fleet of Ultras using the either Ultraview or the alk phos kits.
John PJ Coleman, HT(ASCP)QIHC
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