Hi Carole,
While I have no direct experience with human on human IHC, my first thought
would be to biotinylate your primary antibody and then you would omit the
anti-human secondary which is the most likely source of your background
staining.
If you are using fluorescence you could also
About 20 years ago in my lab they were developing an anti-human melanoma
antibody that we used to test on human melanoma (+) cases and we approached
more or less in the way you suggest and were able to eliminate background.
You just have to start testing, keep good records of what you do and
Carole, Look at Golden Bridge International Labs. They have a human-on-human
detection system.
http://gbi-inc.com/
I've worked with them before and they have excellent products. They also supply
some products to other vendors that are rebadged.
Tim Morken
Supervisor, Electron
